The Jasco J-810 Spectropolarimeter (163-900 nm) is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer. It is designed to allow secondary structure determination by circular dichroism; specifically measuring the left-and right-handed circularly polarized light of optically active molecules. It is equipped with a computer-controlled Peltier device (3-90 °C) and a two-syringe titrator to assess thermal/chemical stabilities of proteins/ polypeptides or to monitor ligand binding and is controlled by Jasco’s Spectra Manager software.
CD applications include:
- Determination of optical purity
- Estimation of protein and nucleic acid conformation
- Determination of the conformational changes of protein and nucleic due to molecular interactions
- Structural comparison between wild-type and mutant proteins
- Determination of the thermodynamically dependent folding and unfolding of proteins and nucleic acids
- Determining the binding constants
- Kinetics of folding and unfolding of macromolecules
- Thermodynamic parameters from temperature dependencies of CD signals
Use and Management
The purpose of this instrument is to support research at Boise State University and surrounding research facilities. First-time users please contact the BRC for information on training and access requirements. Qualified users will be able to book instrument time and use the instrument independently.
Circular Dichroism References:
For rate information please see BRC Recharge Rates found here
Please contact Dr. Lisa Warner at 208-426-2260 or by email at firstname.lastname@example.org for more information.