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Services and Equipment


  • Fiscal Year 18 (October 1, 2017 – June 30, 2018)

    The BRC has three Service Fee Groups:
    BSU-Boise State University users (faculty, staff student)
    Non-Profit- Other Academic or Federally funded users
    Commercial– Commercial for – profit entity users

    *Current as of October 2017: Cost Schedules for all equipment will be reviewed regularly and rates adjusted

    MASS SPECTROMETRY

    DescriptionService ProvidedBSU UserNon-ProfitCommercial
    Tryptic protein digestion
    Sample Preparation
    $8.85/sample
    $12.43/sample
    $20.00/sample
    LC-MS shotgun Proteomics analysis
    Mass Spec Analysis
    $73.66/sample$103.49/sample$200.00/sample
    MS Analysis by direct infusionMass Spec Analysis
    $37.67/sample
    $52.93/sample
    $120.00/sample
    LC_MS analysis of metabolites or intact proteinsMass Spec Analysis$58.20/sample
    $81.77/sample
    $150.00/sample

    IMAGING

    DescriptionService ProvidedBSU UserNon-ProfitCommercial
    Zeiss LSM 510 Meta Confocal
    Microscope - Operation Rate
    Instrument Usage and Training$16.71/hour
    $23.48/hour
    $150.00/hour
    Skyscan 1172 Scanner MicroCT- Operation RateInstrument Usage and Training$85.30/hour$119.85/hour$200.00/hour

    HISTOLOGY

    DescriptionService ProvidedBSU UserNon-ProfitCommercial
    Tissue Embedding System Leica TP1020/Leica EG1150H
    Paraffin Embedding of Tissues$2.24/sample$3.14/sample$50.00/sample
    Paraffin Sectioning Leica RM 2235Thin Sectioning of Paraffin Embedded Tissues$3.20/block
    $0.21/slide
    $4.50/block
    $0.30/slide
    $35.00/block
    $6.00/slide
    Frozen Tissue Sectioning Leica CM1950
    Thin Sectioning of Frozen Tissues$59.54/hour$83.66/hour$125.00/hour
    Thick Tissue Sectioning Leica VT1000
    Thick Sectioning of Fresh & Fixed Tissues$1.01/hour$1.41/hour$50.00/hour
    Autostaining System Leica AutostainerHistological Staining (H&E)$1.54/slide$2.17/slide$32.00/slide

    BIOMOLECULAR CHARACTERIZATION

    DescriptionService ProvidedBSU UserNon-ProfitCommercial
    Jasco J-810 CD SpectropolarimeterInstrument Usage and Training$25.55/hour$35.90/hour$60.00/hour
    Beckman ProteomeLab XL-1 AUCIn-solution Characterization$147.05/run$206.61/run$250.00/run
    Plasmid Propagation from Cell StockSample Preparation $17.57/sample$24.68/sample$50.00/sample
    Plasmid Propagation from Plasmid Stock Sample Preparation$39.37/sample$55.32/sample$75.00/sample
    Soluble Protein Expression TestSample Preparation$136.78/sample$192.18/sample$200.00/sample
    Plasmid MaxiPrep
    Sample Preparation$58.81/sample
    $78.41/sample
    $185.00/sample
    Isotope N15 Label Prep (500ml)
    Sample Preparation$132.10/sample
    $185.60/sample
    $1100.00/sample
    Isotope N15/C13 Label Prep (500ml)
    Sample Preparation$264.10/sample
    $371.06/sample
    $1200.00/sample
    LB Prep (500ml)
    Sample Preparation$86.22/sample
    $121.13/sample
    $1000.00/sample
    Protein Purification (IMAC)Sample Preparation $66.14/sample$92.92/sample$300.00/sample


  • BRC Mass Spectrometry Banner

    Welcome to the BRC Mass Spectrometry Facility!

    Our mission is to provide a wide range of analytical capabilities through fee-for-service in the areas of protein, biological and small molecule research to Boise State University investigators, external academic and industrial partners. If you have any questions regarding our services or wish to know more about mass spectrometry, its capabilities and applications, please contact us:

    Xinzhu Pu, PhD
    Mass Spec Facility Manager
    1910 University Drive
    Boise, ID 83725-1511
    Phone: (208)4262233
    Email: shinpu@boisestate.edu

    Instrumentation
    Fees
    Services


    Instrumentation

    The Biomolecular Research Center currently has two mass spectrometers; a Bruker Daltonics maXis Quadrupole Time-of-Flight, Thermo Scientific Velos Pro Dual-Pressure Linear Ion Trap.  Both instruments are located in the BRC, room 215 in the Mathematics Building.

    Bruker Daltonics maXis Quadrupole Time-of-Flight (Q-TOF) Mass Spectrometer

    Bruker QTOFThe Bruker Q-TOF Mass Spectrometer is a hybrid tandem mass spectrometer with outstanding performance including fast acquisition rate (up to 30 Hz for small molecules, up to 5Hz dynamic for peptides), high resolution (50,000 Full Sensitivity and Resolution), high resolution EIC (0.5 – 1mDa on typical LC peaks), and  excellent sensitivity (1 pg Reserpine >100:1 S/N RMS). This mass spectrometer is coupled with a Dionex Ultimate 3000 HPLC system and an innovative Captive electrospray source. In combination with software tools, including Bruker Compass Data Analysis, Smartformula, ProteinScape, Mascot protein search engine, and Profile Analysis, we use this LC-MS system in small molecule identification, metabolomics analysis and protein characterization.

    Publications and presentations utilizing data from samples analyzed with the Bruker maXis Q-TOF  Mass Spectrometer should include the following acknowledgement:  “This research  was made possible by the National Science Foundation Grant NO: 0923535.”

    Thermo Scientific Velos Pro Dual-Pressure Linear Ion Trap (LIT) Mass Spectrometer

    Velos LIT_2The Themo Scientific Velos Pro Linear Ion Trap Mass Spectrometer offers Trap-HCD (Higher-Energy Collisional Dissociation) combined with CID (Collision-Induced Dissociation), and PQD (Pulsed-Q Dissociation) to enhance coverage and sensitivity of proteomic analysis. An Easy nLCII nano liquid chromatographic system is coupled to the mass spectrometer through a nanoelectrospray source for protein characterization. In combination with the Thermo Proteome Discoverer 1.3, Sequest and Mascot database search engine, this LC-MS system is our work horse for routine proteomic analysis.

    Publications and presentations utilizing data from samples analyzed with the LTQ Velos Mass Spectrometer should include the following acknowledgement:  “This research  was made possible by the INBRE Program, National Institutes of Health Grant NOs: P20 RR016454 (National Center for Research Resources) and P20 GM103408 (National Institute of General Medical Sciences),  by the Idaho State Board of Education’s Higher Education Research Council (HERC) and the Biomolecular Research Center at Boise State University.


    Fees

    We charge for every sample run, regardless of whether an expected result is obtained. The only exception is if the lack of result is caused by human errors or instrument problems in the mass spec facility.

    Analyses are charged at the rates listed below per sample submitted:

    ServicesBSU UserExternal non-profit usersExternal for-profit users
    Tryptic Digestion and Protein Identification (gel band/slice or simple protein mix in solution)$67.49$94.49$230.00
    Protein molecular weight determination$38.33$53.66$160.00
    Small molecule identification by direct infusion$32.79$45.91$150.00
    Metabolites analysis
    by LC-MS
    $40.03$56.04$175.00
    iTRAQ protein quantification by LC-MS$57.93$81.11$240.00

    Services

    We currently provide the mass spectrometry analyses listed below. If you have needs which are not listed below, please contact us to discuss your project with our staff. We will do our best to help you get your research done!

    Mass spectrometry is a powerful tool in protein and metabolite identification and quantification. However, just like many other analytical tools, it has limitations and there are many factors that will affect the results.  We strongly encourage our users to discuss their projects with our facility staff before they submit samples for analysis. We would like to discuss if mass spectrometry is a suitable for your project, the best mass spec workflow for your specific needs, and how to prepare your sample for mass spec analysis. To ensure the success of analysis, samples must be prepared in a way that is compatible with mass spec. Please follow the sample preparation guide to prepare your samples for mass spec analysis.

    Tryptic Digestion and Protein Identification

    Protein identification by tandem MS after protease digestion is a robust tool in proteomics with high successful rates. Samples can be visible bands excised from Coomassie blue or silver-stained gels. Silver staining must be performed with mass-spec compatible reagents,e.g. Pierce Silver Stain Kit for Mass Spectrometry, catalog # 24600. We may refuse submissions stained using MS incompatible methods. We also accept samples in solution which contains purified protein or several proteins. Please contact facility staff before you prepare your sample for in-solution digestion because some of the chemical commonly used in protein extraction are detrimental to mass spec. For complex samples, fractionation is usually required, for example, SDS-PAGE or 2D gel electrophoresis. Protein samples will be digested with protease in gel or in solution and resulted peptides will be analyzed by tandem MS. Trypsin is the most commonly used protease and is the enzyme we routinely use. Some proteins may require other proteases, such as Lys-C, Asp-N, chymotrypsin, etc. If this is case, we can accommodate.

    Guideline for Preparing gel slice/band:

    • Electrophoresis
      • Try to use a 1 mm thick gel.
      • If possible, try using precast gels and pre-made electrophoresis running buffer.
      • We highly recommended to electrophorese a positive control such as BSA or some other known protein that stains to approximately the same level as your unknown protein of interest.
    • Band excision
      • Use a clean scalpel or razor blade (you may wish to first sonicate the blade for 5 min acetonitrile or ethanol in a glass beaker) to excise protein bands/spots of interest from the stained polyacrylamide gel. Excise only the stained part(s) of the band and trim off the non-stained gel as much as possible. Gel band/slice size should be no larger than 5mm in length.
      • Cut each gel slice into small pieces (~1 mm3) and transfer the pieces to a clean, sterile 1.5 mL microcentrifuge tube pre-rinsed with 50% organic solvent, such as methanol and acetonitrile.
      • Also excise and dice a gel piece from a protein-free region of the gel in parallel as a negative control, and your positive control (for example, BSA) if you ran it.
      • Close the tube as soon as your bands are inside. Submit sample to the Mass-Spec facility immediately or store gel slices at -20C for a brief period before submission.
    • Strategies to avoid Keratin contamination

    All humans are constantly shedding skin cells so there is a large amount of keratin in the air of labs, on surfaces, and in gel boxes, glassware etc. that have been left uncovered on benches. Large amounts of keratin in a sample can mask proteins of interest that are less abundant. Although human keratin contamination cannot be completely avoided, it is important to minimize it during each sample preparation step.  Cleanliness is the key. Here are some recommendations:

    • A key factor in avoiding keratin is to avoid contamination with dust particles. Start with a scrupulously clean work surface. If possible, for best dust control during sample prep work such as cutting gel bands or performing in-gel trypsin digestion, try working in a HEPA-filtered laminar-airflow hood. Wipe the inside of the hood and surface of plasticware packs with ethanol.
    • DO NOT handle anything that will come into contact with your protein with your bare hands! Always wear powder-free nitrile gloves (never use latex gloves, as natural rubber contains significant amounts of keratin and other proteinaceous materials). It may be necessary to wear protective clothing during all phases of sample preparation and to cover your head.
    • Try using precast gels, pre-made electrophoresis running buffer.
    • If casting your own gel:
      • Clean the gel plates well with a soapy sponge, rinsing well with ethanol (or bleach overnight).
      • Handle the plates by the edges and wear gloves to avoid transfer of keratin from your hands to the gel plates.
      • Before preparing solutions, wash the glassware well to remove any dust that might have accumulated during storage.
      • When staining gels:
        • Rinse the staining container well before beginning. Also, rinse the gel thoroughly at each stage. Keep the container closed to keep dust out.
        • When cutting gel bands:
          • This is the step where keratin contamination is most likely to occur. If possible, work in a laminar flow hood. Before starting, wipe down the work a wipe down the work area and any tools that will contact the gel band with an ethanol-soaked Kimwipe.

    Prepare sample for in-solution digestion

    Detergents are incompatible with mass-spectrometry and must be avoided. Failure to do so will contaminate the instrument and result in costly and time-consume maintenance and serious down-time for the instruments. Therefore we require users to contact facility staff before preparing in-solution sample for submission to ensure samples are free of mass spec in=compatible reagents.

    • Do not use ANY detergents in ANY step of sample-prep. This includes, but is not limited to: SDS, CHAPS, NP-40, and Triton-X100 (or related).
    • If detergents and other reagents that are not compatible with mass spec must be used to extract protein, precipitation procedure, for example, acetone precipitation can be used to eliminate these interfering reagents.
    • Optimal sample quantity is 20 µg protein in ~20µL solution.

    Protein molecular weight determination

    Molecular weight determination of intact protein can be achieved by direct infusion via a syringe pump or Liquid Chromatographic (LC) – Electro Spray Ionization (ESI) on our Bruker maXis Q-TOF. A concentrated solution of desalted protein is sprayed into the instrument. . Ion signals that correspond to different charge states of the intact protein molecule are recorded. This data is then deconvoluted to obtained protein molecular mass, typically up to 200kDa, depending on the homogeneity of the sample.

    HPLC-MS is the recommended method because it removes salts and other interfering compounds and enhances signal to noise ratio. This is the method we routinely use to determine the molecular weight of intact proteins. However, non-covalent protein complexes cannot be measured with HPLC because they require 100% aqueous conditions at neutral pH value. In this case, direct infusion via a syringe pump is the method of choice.

    Sample preparation guide:

    • Samples should be free of detergents and viscous solvents, including DMSO, DMF, and THF.
    • Trifluoroacetic acid (TFA) suppresses ionization, and should be kept below 0.1%. Commonly used alternatives to high concentrations of TFA are mixtures of either 1% acetic or 0.1% formic acid with 0.025% TFA
    • Because LC cannot be used to desalt samples, non-volatile buffers should be avoided when analyzing non-covalent protein complexes. A recommended solvent for this sample type is 10 mM ammonium acetate solution in water. Other protein sample, the recommended solvent is 1% formic acid and 25-50% acetonitrile in water. Since LC will be used to clean up salts and other contaminants for this type of samples, non-volatile salts can present in the sample. However, concentration should be kept as low as possible.
    • Glycerol should be limited to no greater than 1%.
    • Sample concentration: Good results can be obtained with 2.5 µM at 5 kD, 5 µM at 20 kD, 10 µM at 40 kD, and 25 µM at 60 kD.
    • Sample volume: 20-50 µL for HPLC and 50-100 µL for direct infusion.

    Small molecule identification by direct infusion

    We provide accurate mass analysis of small molecules in relatively pure samples. This analysis will be carried out on our Bruker maXis Q-TOF.

    Sample preparation guide:

    • In general, volatile low MW protic solvents are preferred. Methanol or water-methanol solution is the typical dilution solvent. Isopropanol and acetonitrile-water mixtures are also acceptable solvents. Pure water or pure acetonitrile are not good solvents for electrospray ionization. If water is required for solubility, up to 50% water may be added. Try to use the highest purity solvents including water, preferably MS grade or HPLC grade.
    • High MW or viscous solvents should be avoided, including DMSO, DMF, and THF.
    • Hydrocarbon solvents, such as hexane and benzene, are not amenable to ESI.
    • Acetone may be used but is not preferred, as even high-grade acetone typically contains contaminants which show up as strong peaks in the MS, and may overwhelm the analyte signal.
    • If the sample contains TFA (e.g. from a reverse-phase HPLC run), remove the TFA by lyophilization or by drying down the sample overnight under high vacuum.
    • Please be sure to indicate if the compound is sensitive to acid or basic conditions, as small amounts of acid (formic, acetic) or base (ammonium hydroxide, triethylamine) are often added to samples in order to enhance ionization. This information will also help determine which MS polarity should be used.
    • In general, 50-100 µL of 1mg/ml sample is more than enough for analysis. Largely due to differences in the ionization efficiency, sensitivity for different compounds may vary. Our instruments are capable of detecting down to the 1 µg/mL level. However, it is good practice to submit around 1 mg/mL.
    • All samples must be filtered through 0.22 µM filter as the particles in sample may clog the spray needle.
    • Please specify if compound may become unstable under certain conditions.

    Metabolites analysis by LC-MS Instrumentation

    We provide quantitation of small molecular metabolites. This measurement is carried on our Bruker maXis Q-TOF coupled with a Dionex Ultimate 3000 LC.

    Sample Preparation for L-MS analysis of metabolites

    • Samples should be dissolved in water-miscible solvents. Optimal solvent is the one with similar strength to LC mobile phase at the start of the LC gradient. Try to use the highest purity solvents including water, preferably MS grade or HPLC grade.
    • High MW or viscous solvents should be avoided, including DMSO, DMF, and THF.
    • If the sample contains TFA (e.g. from a reverse-phase HPLC run), remove the TFA by lyophilization or by drying down the sample overnight under high vacuum.
    • Please be sure to indicate if the compound is sensitive to acid or basic conditions, as small amounts of acid (formic, acetic) or base (ammonium hydroxide, triethylamine) are often added to the LC mobile phase in order to enhance ionization. This information will also help determine which MS polarity should be used.
    • Sample volume: In general, 50-100 µL of sample is enough for analysis.
    • All samples must be filtered through 0.22 µM filter as the particles in sample may clog LC autosampler needle and column.
    • Please specify if compound may not be stable under certain conditions.

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  • Skyscan

     Skyscan banner images Jan2013

    BRC Skyscan 1172 Micro-CT Scanner

    The Biomolecular Research Center’s Skyscan 1172 Micro-CT Scanner is located in room 213 in the Mathematics building on the campus of Boise State University.  The Skyscan 1172 scanner, with an X-ray source of 20-100V, 10W, <5 µm spot size or 20-80V, 8W, <8 µm spot size, has a fully distortion-corrected 11Mp X-ray detector that is a 12-bit cooled CCD fiber-optically camera coupled to scintillator.  The detail detectability of the Skyscan 1172 ranges from 0.8/1.0 µm (when operated at the highest resolution) to 25 µm. The Skyscan 1172 currently has two filter choices that can be set into 3 positions to allow for scanning high-density tissues such as bones to low-density softer tissue such as lung. The maximum object size, or field of view, is 50 mm in either diameter or height when scanner is run in offset mode. The Skyscan 1172 with dynamically variable acquisition geometry provides the shortest scan times possible at any magnification and cross-section images are generated in a wide range of formats up to 8000 x 8000 pixels.  2D/3D reconstruction can be performed using a single PC or using the 4-PC cluster in the BRC facility to enhance post-scan reconstructions times.

    Use and Management
    The purpose of this instrument is to support research at Boise State University and surrounding research facilities and is operated as a recharge instrument at an hourly rate. Scan times are dependent on specimen size and resolution parameters.

    Contact Information
    Raquel Brown
    BRC Manager/Confocal Microscope System
    (208) 426-2125
    rbrown@boisestate.edu

    Publications and presentations utilizing data from samples imaged with the Skyscan MicroCT should include the following acknowledgement:  “This research was made possible by support from the Idaho State Board of Education’s Higher Education Research Council (HERC) and the Biomolecular Research Center at Boise State University.”

    Zeiss

    Confocal banner images Jan2013

    BRC Zeiss LSM Meta 510

    The Biomolecular Research Center’s Zeiss Microscope, LSM 510 Meta confocal imaging system is located in room 213 of the Mathematics building on the campus of Boise State University.  The Axiovert Observer Z1 inverted microscope has filter sets appropriate for DAPI, FITC and CY3/TRITC to view samples in a conventional widefield microscopy mode as well as the objective lens: 10x, 20x, 40x, 63x and 100x.  The LSM Meta 510 is configured with six excitation lasers lines (405, 458, 488, 514, 543 and 633 nm), two photomultipliers, a detector for bright field image acquisition and a polychromatic META detector capable of detecting many standard fluorophores, including DAPI, FITC, CFP, YFP, Rhodamine, TRITC, Cy3, Cy5, Texas Red  and many AlexaFluor dyes.  Excitation dichroics and emission filters are operated via the LSM software. Added accessories include a heated stage chamber and objective warmer from PECON for live cell experiments and an AxioCam MRm monochrome digital camera.

    Use and Management
    The purpose of this instrument is to support research at Boise State University and surrounding research facilities.  The instrument is managed by Raquel Brown. Users with substantial needs for the confocal microscope will be trained so they can use the system unassisted.  New users will have a 3-4 hour introduction that covers start-up, computer software introduction for basic confocal system operation, basic image manipulation, data storage, and clean-up/shut-down procedures. In a follow-up session, the potential user will demonstrate that he/she can safely and competently operate the instrument. With this, the user will have allowable unsupervised access to the instrument at the confocal system hourly rate. There will be a discounted hourly rate available for long-term experiments that utilize the scope after hours.
    Users, who have simple or solitary studies and who do not want to be formally trained can elect to have the system operated by Raquel while providing investigator feedback for the price of confocal hourly rate plus a consulting fee.

    LabImaging Software on Monitor

    Contact Information
    Raquel Brown
    BRC Manager/Confocal Microscope System
    (208) 426-2125
    rbrown@boisestate.edu

    Publications and presentations utilizing data from samples imaged with the  Zeiss LSM Meta 510 confocal microscope should include the following acknowledgement:  “This research  was made possible by the INBRE Program, National Institutes of Health Grant NOs: P20 RR016454 (National Center for Research Resources) and P20 GM103408 (National Institute of General Medical Sciences) and the National Science Foundation Grant NO: 0619793. “

  • Welcome to the BRC Histology Facility!

    Our mission is to provide access to a wide range of histological services for Boise State investigators, external academic investigators and industrial partners.  We can provide direct services, training and/or access to equipment for embedding, sectioning and staining of various tissue specimens.  We are a fee-for-service center, and use of our facility requires users to establish an ilabs account.  To establish an account, or to ask questions regarding our equipment and/or services, please contact:

    Cindy Keller-Peck, PhD
    Histology Facility Manager
    Biomolecular Research Center
    1910 University Drive
    Boise, ID 83725-1511
    Phone: (208) 426-2254
    Email: CKPeck@boisestate.edu

    INSTRUMENTATION
    SERVICES
    SAMPLE SUBMISSION


    Services

    Paraffin Embedding 

    Leica TP1020 + EG1150 150px

     

    The BRC uses a Leica TP1020 benchtop tissue processor to dehydrate, clear and infiltrate tissues with paraffin.  Embedding is done with the aid of a Leica Tissue Embedding Center.   Completed blocks will fit in most standard microtome specimen clamps.  We currently do not offer training for this equipment.

     

     

    Paraffin Sectioning

    Leica RM2235 150px

    The BRC uses a Leica RM2235 manual rotary microtome to section blocks of paraffin embedded tissues.  Most sections are cut at 8 mm, but the microtome has a sectioning range of 1-60 mm in thickness.  We can section tissue regardless of whether it was embedded in our facilities.  The microtome is available for independent use.  We will train users who wish to learn about the technique or would like to section their own tissues.

    Frozen Sectioning

    Leica CM1950 150px x 150px

    Tissues for frozen sectioning can be prepared according to investigator needs.  Fresh tissues are generally snap frozen in OCT using liquid nitrogen/isopentane.  Fixed, cryoprotected tissues may be frozen using liquid nitrogen or dry ice.  The BRC currently uses a Leica CM1950 clinical cryostat for sectioning frozen tissues.  It has a sectioning range of 1-100 mm.  The cryostat is available for independent use.  If requested, we will train users to freeze and section their own tissues.
    Vibratome Sectioning

    Leica VT1000 150px

    Thick sections of tissues are cut on the Leica VT1000 vibratome.  It has a sectioning range of 10-999 mm.  Additional adjustable parameters include blade clearance angle, frequency, amplitude and sectioning speed.  Tissues with a high degree of structural integrity may be sectioned without a support medium.  In instances where a tissue requires support, tissue may be embedded in low melting point agarose. The vibratome is available for independent use. If requested, we will train users to embed and section their own tissues.

    Staining

    Leica autostainer XL 150px

    The histology facility currently offers staining with the following histological dyes:  Hematoxylin & Eosin, Alcian Blue, and Sirius Red.  New dyes and immunohistochemistry services are being added as needed, please email the facility for special requests.   We are not currently training investigators to use the Leica Autostainer.  However, we will train interested parties in staining techniques to be used back in their individual laboratories.


    Service Equipment Training Available
    Paraffin EmbeddingLecia TP1020/Leica EG1150 embedding equipmentNo*
    Paraffin SectioningLecia RM2235 MicrotomeYes
    Frozen SectioningLeica CM1950 CryostatYes
    Vibratome SectioningLecia Vibratome VT1000Yes
    StainingLeica Autostainer XLNo*

    For rate information please see Histology BRC Recharge Rates
    *May be available by special request.


    Sample Submission

    Users who wish to submit samples for processing must first obtain an iLabs account.  A one-time registration is required to place service requests or reserve equipment. Please visit the  iLabs registration page:  https://boisestate.ilabsolutions.com/account/login.  For questions on using iLabs to set up histology services, please contact the Histology Facility Manager.

    Once an iLabs account is established, and a service request or equipment reservation is made, tissue should be delivered to the Histology Lab in room 211 of the Math building.  The tissue should be accompanied by a completed copy of the BRC Histology Request Form. This form may also be filled out and emailed to CKPeck@boisestate.edu.

    Tissue Preparation:  Tissues are most commonly submitted to the BRC in 10% neutral buffered formalin (NBF).  However, tissues are accepted in other fixatives provided the researcher feels it is the most appropriate way to maintain the molecular and macromolecular aspects under investigation.

  • Biomolecular Characterization Facility

    Our mission is to provide access to biomolecular characterization services for Boise State investigators, external academic investigators, and industry partners. We can provide direct services, training and/or access to equipment for plasmid propagation, rapid protein solubility screen, circular dichroism and analytical ultracentrifugation. We are a fee-for-service center and use of our facility requires users to establish an ilabs account for reservations. To establish an account, or to inquire about our services and equipment, please contact:

    Lisa Warner, PhD
    Biomolecular Characterization Manager
    Biomolecular Research Center
    1910 University Drive
    Boise, ID 83725-1511
    Phone: (208) 426-2260
    Email: lisawarner@boisestate.edu


    SERVICES

    Protein Expression and Purification
    The first step in biomolecular characterization usually requires recombinant expression of proteins of interest. At the BRC, we provide services to propagate plasmids as well as a rapid, small scale screen for soluble protein expression in E. coli using up to 4 expression strains including basic BL21(DE3) and derivatives that can increase soluble expression of proteins with cell toxicity (e.g. enzymes), and with disulfide bonds, and from plasmids with rare codon usage.

    Sample requirements

    • Plasmid propagation: Cell stock (e.g. plasmids from Addgene) or plasmid stock.
    • Small scale solubility screen: Expression plasmid with T7 inducible promoter.

    Circular Dichroism
    The BRC houses a Jasco J-810 Spectropolarimeter (163-900 nm). This hybrid instrument consists of a variable wavelength spectropolarimeter and absorption spectrophotometer. It is equipped with a computer-controlled Peltier device (3-90 °C) and a two-syringe titrator, which can be used to assess thermal/chemical stabilities of proteins/polypeptides or to monitor ligand binding.

    CD applications include determining:

    • Optical purity of substance
    • Protein and nucleic acid secondary and tertiary structure
    • Conformational stability at varying temperature, pH, or denaturant concentrations
    • Conformational changes due to molecular interactions
    • Conformation of mutants or proteins expressed in different systems
    • Thermodynamics binding constants
    • Kinetics of folding and unfolding of macromolecules

     Buffer Requirements

    • Buffers that absorb in the spectral region of interest should be avoided.
    • 10 mM sodium or potassium phosphate buffer is generally recommended for CD. Tris or TEA can be used but should be pH’d with sulphuric or phosphoric acid.
    • Many buffers absorb at the shorter wavelengths where most of the structural information of interest is found. AVOID chloride, citrates, MOPS, imidazole and DTT.
    • Record a spectrum of the buffer alone before starting with samples to ensure the buffer absorbance is not a problem.

    Sample Requirements:

    • The sample should be as pure as possible since impurities will contribute to the CD signal. Dialyzing into the buffer of choice is highly recommended.
    • The sample concentration is determined by the pathlength of the cuvette. The BRC has several choices.
      • A starting guideline is the sample should not exceed an absorbance of 0.9 OD over the wavelengths you want to scan. Measure the absorbance of your sample at all of the wavelengths you plan to use in your experiment to ensure your sample does not exceed 0.9 OD.
      • When using a 1 mm cuvette, 0.2 mg/ml for the 190-230nm range is a good starting estimation.
    • A smaller pathlength cuvette will decrease solvent absorbance to allow scanning down to lower wavelengths but will require a more concentrated sample.
    • Bring at least 500uL of sample and at least 10mL of identical buffer, ideally from dialysis.

    Use and Management
    The purpose of this instrument is to support research at Boise State University and surrounding research facilities. First-time users please contact the BRC for information on training and access requirements. Qualified users will be able to book instrument time and use the instrument independently.


    Analytical Ultracentrifugation
    Analytical ultracentrifugation relies on the simple premise that biomolecules in solution will sediment in the presence of the gravitational force applied by the centrifuge. The rate at which the particle sediments will be affected by its molecular weight, size and shape allowing one to determine these parameters experimentally. There are two basic types of experiments performed with the analytical ultracentrifuge, sedimentation velocity and sedimentation equilibrium.

    • Sedimentation velocity experiments rely on a high angular velocity which causes the solute to sediment rapidly leading to a depletion of solute near the meniscus. A boundary forms between the depleted and uniform concentration areas of the solute which can be monitored to determine the sedimentation coefficient which is a measure of the effective size of the solute.
    • Sedimentation equilibrium experiments employ a smaller angular velocity than sedimentation velocity which causes the solute to sediment at a much slower rate. As sedimentation occurs, diffusion opposes the gradual concentration increase in the bottom of the cell. After the two opposing forces reach equilibrium, the diffusional flow exactly balances the sedimentation flow leading to a concentration profile that is constant over time from which the molecular weight of the solute can be determined.

    AUC applications include determining:

    • Sample purity
    • Molecular weight
    • Oligomer formation
    • Conformational changes
    • Ligand binding

    Instrument Information:

    • XL-I (Beckman Coulter, Installed)
    • Rotors:
      • An-60 Ti – 4-place titanium rotor rated for 60,000 rpm
      • An-Ti 50 – 8-place titanium rotor rated for 50,500 rpm

    Sample Requirements:
    Sedimentation Velocity: At least 500uL of sample with an OD of 0.7-0.8 at the absorbance wavelength.
    Sedimentation Equilibrium: At least 500uL of sample with an OD of 0.2-0.3 at the scanning wavelength.
    **Bring at least 10mL of buffer as well.

    Use and Management
    The purpose of this instrument is to support research at Boise State University and surrounding research facilities. First-time users please contact the BRC for information on training and access requirements. Qualified users will be able to book instrument time and use the instrument independently.

    For rate information please see BRC Recharge Rates found here.
    Please contact Dr. Lisa Warner at 208-426-2260 or by email at lisawarner@boisestate.edu  for more information.

    Jasco

    JascoImage

     

     

    The Jasco J-810 Spectropolarimeter (163-900 nm) is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer. It is designed to allow secondary structure determination by circular dichroism; specifically measuring the left-and right-handed circularly polarized light of optically active molecules.  It is equipped with an equipped with a computer-controlled Peltier device (3-90 °C) and a two-syringe titrator to assess thermal/chemical stabilities of proteins/ polypeptides or to monitor ligand binding and  is controlled by Jasco’s Spectra Manager software.

    CD applications include:

    • Determination of optical purity
    • Estimation of protein and nucleic acid conformation
    • Determination of the conformational changes of protein and nucleic due to molecular interactions
    • Structural comparison between wild-type and mutant proteins
    • Determination of the thermodynamically dependent folding and unfolding of proteins and nucleic acids
    • Determining the binding constants
    • Kinetics of folding and unfolding of macromolecules
    • Thermodynamic parameters from temperature dependencies of CD signals

    Use and Management
    The purpose of this instrument is to support research at Boise State University and surrounding research facilities.  First-time users please contact the BRC for information on training and access requirements. Qualified users will be able to book instrument time and use the instrument independently.

    Boise State University users – faculty, staff, students
    Non-profit – Other academic or federally funded users
    Commercial – For profit users

    Circular Dichroism References:

    UCI Department of Chemistry Introduction to circular dichroism
    Circular Dichroism_Greenfield 2004
    J-810 Series Hardware Function Manual


 

DNA and Protein Isolation and Characterization

Beckman Optima TL UltracentrifugeThe Beckman Optima TL Ultracentrifuge is an ultracentrifuge with features that include thermoelectric refrigeration, microprocessor control, very smooth acceleration/  deceleration profiles, air cooled, imbalance tolerant drive, fast instrument set-up and diagnostic displays. This system can store up to 10 user programs, can reach speeds of 100,000 rpm and can generate a force of 543,000 x g max.


Beckman L8-750 UltracentrifugeThe Beckman L8-70M Ultracentrifuge has been the mainstay of laboratories world-wide for ultra high speed centrifugation processes. The ultracentrifuge can operate at much higher speeds and allows for the faster separation of samples. Some example applications include: sucrose gradient isolation of proteins, density gradient fractionation of lipoproteins, fractionating subcellular components (nucleosomes, microsomes, mitochondria, etc.), plasmid purification in CsCl, and RNA pelleting in CsCl. Two rotors are available: (1) Type 45 Ti: 6 x 94 mL samples, max speed 45,000 rpm (235,000 x g) or (2) Type 70 Ti: 8 x 39 mL samples, 70,000 rpm (504,000 x g).


Agilent 1200 SeriesThe Agilent 1200 HPLC series can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture. This HPLC is a preparative autosampler designed to perform preparative LC. It allows injection of sample volumes from µL to mL ranges from vials.


Agilent 1100The Agilent 1100 HPLC is a stand-alone system that supports both ultraviolet and fluorescence detection. The HPLC pump is a binary system capable of up to a 10 ml/min flow rate and 400 bar of pressure. The UV-vis detector is a diode array, with a wavelength range from 190 – 950 nm. The fluorescence detector provides simultaneous quantitative and qualitative information for up to four signals simultaneously. The system also incorporates a mobile phase degasser, column heater, and 100 vial autosampler. Data acquisition and analysis is performed using ChemStation software.


Trace-GC-UltraThe Thermo Scientific TRACE GC Ultra multi-channel gas chromatograph was developed for the evolving requirements of modern laboratories. With the most complete range of proprietary inlets, sensitive detection systems, smart accessories, and ancillary devices, the Ultra platform features unique technologies that raise the standards of speed, sensitivity and separation in gas chromatography. Automated features increase sample throughput with analysis performed quickly without the loss of precision.


SORVALL ST16RThe Thermo Scientific Sorvall ST 16R refrigerated centrifuge is a general purpose centrifuge perfect for everyday sample processing, including clinical protocols, cell culture applications and microplate processing. This unit has a 4 x 400ml capacity with a maximum speed/RCF of 15,200rpm/25.830 x g. The refrigerated version is ideal for handling temperature-sensitive sample processing between -10° and +40°C.


Sorvall Legend X1RThe Thermo Scientific Sorvall Legend X1R refrigerated centrifuge can handle a range of general-purpose processing including cell culture applications, microplate, bioproduction, blood separation, microbiology and others. This unit has a 1L capacity with a maximum speed/RCF of 15,200rpm/25.830 x g; ideal for handling temperature-sensitive sample processing between -10° and +40°C.


Accuri C6 Flow cytometerThe BD Biosciences Accuri™ C6 is a personal flow cytometer is easy to use, simple to The system is equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use.


Luminex 100The Luminex 100 is a compact lab analysis system based on the principles of flow cytometry which enables the simultaneous assay of up to 100 analytes in a single well of a microtiter plate with very small volumes. This system delivers fast and cost-effective bioassay results on many assay formats including nucleic-acid assays, receptor-ligand assays, immunoassays and enzymatic assays.


ECM 630The BTX ECM 630 Electroporator is ideal for gram positive and gram negative bacteria, yeast, fungi and other microorganism transformation in HV mode, optimized for a broad range of mammalian cell lines in LV mode. Applications include, yeast, insect cells, bacteria, cDNA libraries, BAC library; novel applications include transdermal drug/gene delivery and intact plant gene delivery.


ECM 830The ECM 830 is a Square Wave Pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.


Nucleofactor deviceThe Nucleofector™ Device is the single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format. The Nucleofector™ II/2b Device can also be used for bacteria transformation.


OMNI HomogenizerThe OMNI General Laboratory Homogenizer is a powerful versatile and durable homogenizer that can handle virtually any processing application. It has a high powered motor that can handle even the toughest tissue samples, and emulsion and dispersions are quickly and easily completed. The OMNI GLH is compatible with stainless steel probes, has a processing range from 200µL to 10L, and can be hand-held or mounted for lengthier processing applications.


Microinjector EppendorfThe Eppendorf InjectMan® NI 2 Micromanipulator  has a menu-controlled, programmable micromanipulator, the InjectMan NI 21 is especially suitable for microinjection into adherent cells. The electronic connection of the InjectMan NI 2 and the FemtoJet®2 or FemtoJet express ensures a very rapid and safe microinjection. The semiautomatic microinjection allows a coordinated process of manipulation and injection: first, the injection parameter is set on the FemtoJet; second, the injection level is fixed via pressing a button on the InjectMan NI 2. The exact axial injection movement ensures a minimum mortality rate, and its high speed facilitates the penetration of rigid structures.


NuAire Tissue Cultrue IncubatorThe NuAire DH AutoFlow Tissue Culture CO2 incubator uses a microprocessor based, non-dispersive infrared CO2 sensor to maintain a very accurate CO2 control is delivered within the incubation chamber.


NuAire Class II, Type A2The NuAire Class II, Type A2 Biological Safety Cabinet is personnel and product protection reducing the potential for exposure to airborne biological or particulate chemical agents in low to moderate risk-hazard research and drug preparations.  The cabinet’s airflow is 30% exhausted / 70% re-circulated to minimize cross-contamination. The BioSaftey Cabinet controls include a minihelic pressure gauge, motor voltage regulator, audible window alarm, main and outlet power circuit breakers, and power switches for exterior mounted fluorescent lights ,  interior outlets, and blower motor.


Rotary Cell Culture SystemThe Rotary Cell Culture System from Synthecon is unique system that provides a low turbulence, low shear culture environment for 3-dimensional cell and tissue structures. Biological structures are suspended in a rotating vessel, providing a dynamic, yet gentle environment that promotes the mass transfer of nutrients and molecular compounds throughout the cell population.


BioRad Mini-PROTEANThe BioRad Mini-PROTEAN® cell is ideal for vertical mini gel electrophoresis. This electrophoresis cell accommodates one or two handcast gels. The Mini-PROTEAN has a casting frame that ensures precise alignment easily accessible side by side casting stand with spring loaded lever tight seal. This system allows for the flexibility of handcast gels traditional SDS-PAGE, Electrophoretic mobility shift assays and Zymography.


BioRad Criterion DodecaThe BioRad Criterion Dodeca cell has the capacity to run up to 12 handcast of Criterion precast gels simultaneously or 11cm ReadyStrip IPG strips to give the optimal combination of separation and speed in 2-D electrophoretic applications.


XCell SureLockThe XCell SureLock™ Mini-Cell Electrophoresis NuPAGE Novex System from Invitrogen is one of the systems available in the lab for electrophoresis. XCell lines of mini-cells are easy to assemble, sturdy and convenient. Currently we have 10 XCell tanks and they are utilized with NuPage precast polyacrylamide gels available cast with 10 or 15 wells, in 1.0mm or 1.5 mm thickness. These 4-12% gels are optimized with a neutral pH that minimizes protein modifications making them ideal for sequencing, mass spectrometry or other analysis where protein integrity is crucial.


iBLOT Blotting SystemThe iBlot® 7-Minute Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes offering the convenience of a bufferless, plug-and-play system.


SNAP ID WESTERNMillipore SNAP I.D Protein Detection System allows researchers to produce high quality western Blots 80% more quickly than conventional immunodetection protocols due to a vacuum-driven technology and built-in flow distributor that drives reagents through membranes and ensures even distribution. This unit is compatible with all membrane types and detection methods, such chemiluminescence and fluorescence.


AGFA 1000 Film ProcessorThe AGFA CP1000 Automatic Film Processor is a compact and lightweight film processor ideal for low-volume processing.  The processor has a fast start up and is easy to maintain. With its simple and accessible film transport system and an infrared dryer instead of a typical hot-air dryer, the system has an efficient short film processing time.


BioTekELx405R 96 wellplate washerThe BioTek ELx405R 96-well Plate washer is designed for applications from basic ELISA to cell and bead washing.  This unit features an overfill protection, unique fluid/flow detection, crosswise aspiration and a built-in positive displacement pump. It has easy to use software provides up to 75 programs, up to 10 cycles/wash and control of dispense height. Aspiration height and delay, soak, shake, aspiration rate and horizontal aspirate and dispense position.


GyromaxThe GYROMAX™ 787 and 787R orbital incubators have a working temperature range from 5°C above ambient to 70°C and the shaker rotates at speeds from 25 to 400 RPM – digitally set. Each incubator chamber includes both a shaking platform that can accommodate a variety of different size flasks up to liters. Each chamber also has a shelf allowing both plates and rotating cultures to incubate simultaneously.


BioRad Biologic LPThe BioRad BioLogic LP low-pressure chromatography is used for biomolecule purification. The system includes both 254 nm and 280 nm filters for nucleic acid and protein detection, and a conductivity cell to monitor gradient progress. The BioLogic houses a peristaltic pump with a flow rate range of 0.05-40 ml/min and a maximum backpressure of 30 psi. The system is compatible with Econo-Column low pressure chromatography columns and low-pressure chromatography media. Tme can collect into eighty 13 x 100 mm tubes or microtubes using a fraction collector. LP Data View software captures data, multitasks, and prints data. Further the system stores up to 50 methods, each method can include 50 pump steps and 50 fraction collection steps.


BioRad Protean IEFThe BioRad PROTEAN IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip.


Thermo Hybridization OvenThe Thermoscientific Hybridization Oven has a 10-bottle capacity rotisserie with an accurate and uniform temperature control and uniformity. Temperature Range from 8 to 85 °C, temperature uniformity within bottle is ±0.25 °C and rotisserie speed from 5 to 15 rpm.


ThermoMix500The ThermoMix 500 is a digitally controlled Peltier heater and cooler (6C – 105 C) orbital mixer (300-1500 rpm) for cell killing assays, RNA transcription, immunoprecipitation, enzyme kinetics, ligations, and more. Five interchangeable sample blocks are available for plates and different size tubes.


Fisher Scientific Accuspin 17RThe Fisher Scientific Accuspin 17R is a high-speed refrigerated micro-centrifuge with a capacity of  48ml (24 x 1.5ml or 2.0ml microcentrifuge tubes, a maximum speed force of 13, 300 rpm/17,000x G, an easy-to-uses controls and digital display, an adjustable 99-min timer and quick-spin feature for short runs.


Eppendorf 5430RThe Eppendorf 5430R Refrigerated Centrifuge is a unique cross-over bringing multipurpose and potential versatility to every lab bench with spin rotors for microcentrifuge tubes and PCR strips.  Additional rotors can accommodate microplates as well as 15ml and 50 ml conical tubes. This unit is equipped with a programmable pre-cooling time, ECO shut-off function and assures accurate temperature control.


ALC PM140The ALC PM140 is a refrigerated centrifuge equipped with a swinging bucket rotor with interchangeable buckets addressing different tube sizes. This unit is ideal for large volume sample processing (48 x 15ml conical, 24 x 50ml conical), has two braking rates, a 30 minute timer and hold.


Nanodrop ND-1000The Nanodrop ND-1000 is sample retention system designed to measure small volume samples of nucleic acids and proteins without the use of cuvettes or capillaries. Absorbance is captured and measured in a sample column made between two optical pedestals, displayed on an easy to read software interface, collected in an exportable spreadsheet and stored within the computer’s hard-drive. Measures DNA, RNA (A260) and Protein (A280) concentrations and sample purity (260/280 ratio); Large concentration range (2 ng/µL – 15,000 ng/µL dsDNA) without dilutions. Nanodrop software is pre-configured methods for common applications such as Nucleic Acid, Protein A280, Microarray, Proteins & Labels, Bradford, BCA, Lowry and Pierce 660nm


Nanodrop 2000CThe NanoDrop 2000C is a spectrophotometer that combines microvolume pedestal technology and cuvette capability. The NanoDrop 2000C is a complete UV-Vis spectrophotometer preconfigured for all common nucleic acid and protein concentration and purity measurements, can measure very low and high concentration samples (0.4-15,000 ng/ μl and with samples as small as 0.5 µL . Cuvette capability allows for kinetics (time or time/ temperature) and cell culture (OD600) measurements. Cuvette position also includes temperature control (37 ºC) and stirring.


GeneQuantThe GeneQuant  is a small, compact spectrophotometer, It has been designed to measure absorbance at 230, 260, 280, 320, 546, 562, 595 and 600 nm, It can determine the concentration of the sample in a variety of units. The GeneQuant is able to measure 1 μl samples without dilutions of cuvettes allowing the sample to be recovered. This machine can be utilized for measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD.


Eppendorf VacufugeThe Eppendorf Vacufuge  is a concentrator available for the efficient and gentle concentration of samples like RNA/DNA and proteins. The system is equipped with a vapor condenser purifying 85% of the exhaust. Two stackable 48-sample rotors are available allowing for the concentration of 96 samples at one time.


Branson 1510 Ultrasonic CleanerThe Branson Tabletop Ultrasonic Cleaner provides a safe and consistent method of cleaning electronics and machine parts when soaking and steaming are insufficient. Touch panel allows for the easy setting and monitoring of cleaning cycles up to 99 minutes at 80 °C


Isotemp RefThe Isotemp Plus Lab Refrigerator was designed for chromatography needs, with two capped access portholes to permit the insertion of thermocouples or other wiring without disturbing the temperature settings. Pushbutton lights and circuit breaker-protected electrical receptacles simplify equipment operation and allow observation of ongoing separations. Unit features microprocessor-controlled thermostats, a digital temperature display and a working temperature range from 1°C to 12°C.


Harvey autoclaveThe Harvey SterileMax is a benchtop sterilizer features a 12” diameter x 18” deep  (1.2 cu.ft.) sterilization chamber.  This sterilizer is easy to set up – by simply adding water, plugging it in and choosing from 4 pre-set cycles, the system is ready to go. This unit also has three safety locks ensuring the chamber will not open until it has depressurized.


Cryo Tank Locator JrThe Thermo Scientific Locator JR Cryo preservation tank is designed with a double-walled, vacuum insulated, narrow mouthed neck to minimize liquid nitrogen loss; maintains temperature uniformity at below -180° with less that 5cm remaining in vessel. Four stainless steel racks, each holding 1-1/4 square boxes, are arranged in numbered slots for easy retrieval; giving the Locator JR the capacity to hold 1600 vials.


Sorvall-Lynx-Ultracentrifuge The Thermo Scientific Sorvall LYNX super-speed refrigerated centrifuge designed to run 24,000rpm/68,905 xg performance with  the maximized capacity of 4 liters for bottles, tubes or microplates. This set up with two Fiberlite carbon fiber rotors, has shorter run-times, increased security and safety features. This system has an intuitive touch-screen control, can hold up to 99 programs and has user access control and password protection to accommodate a range of users.


microcalorThe MicroCal VP-ITC is an ultrasensitive isothermal calorimeter designed to operate through a software interface to minimize operator involvement and facilitate quick, accurate analyses, while eliminating the need for thermodynamic expertise. The capabilities of the isothermal calorimetry as a technique in combination with VP-ITC can be utilized in applications that include the characterization of molecular interaction of small molecules, proteins, antibodies, nucleic acids, biomolecules and lipids and the assessment of the effect that changes in molecular structure has on binding mechanisms and the associated impact on biological activity.


GreenChem Plus mini-Vessel Accessory Set Solvent Extraction Option Perform up to 14 reactions simultaneously with CEM’sGreen Chem™ vessels. These 100-mL vessels are available with either PFA Teflon or glass liners.


Barocycler NEP2320 The compact and versatile Barocycler NEP2320 fills an important need for all laboratories that want improved sample preparation in genomics, proteomics, transcriptomics, and metabolomics. The NEP2320 is a safe and versatile sample preparation instrument designed to use a minimum amount of laboratory bench space, while still providing…the Power of PCT. The mechanisms of action of the PCT SPS differ significantly from other sample preparation methods. Specifically, lysis occurs when samples are subjected to alternating cycles of ambient and high pressure, up to 35,000 psi (45 kpsi Option Available), in the hydrostatic reaction chamber of the Barocycler. The NEP2320 uses available laboratory compressed air, bottled compressed air, or a standalone compressor to create high and ultra-high hydrostatic pressure inside the Barocycler reaction chamber. Exquisite control of the PCT process is accomplished by specialized components, a programmable controller, and software. The NEP2320 quickly and efficiently processes one sample at a time. Although smaller than the NEP3229, the NEP2320 still provides the user with many of the features of the Barocycler NEP3229, including temperature control.

Electrophysiology

Electrophysiology Measurement SystemThe Electrophysiology Measurement System is located in the Integrated Nanomaterials Laboratory, room 2112 of the Environmental Research Building on the Boise State campus. It consists of the MultiClamp 700B amplifier and the Digidata 1550 digitizer. The MultiClamp 700B is a computer controlled microelectrode amplifier designed for patch voltage- or current- clamp measurements of single ion channels, whole-cell voltage- and current-clamp recordings, intracellular electrode recordings, extracellular field potential recordings in an ion channel, neuroscience, nanopore and voltammetry studies. It is interfaced with a Digidata 1550 low-noise, high-resolution 16-bit data acquisition system for digitizing electrophysiology recordings. The two systems are controlled via pCLAMP 10.4 electrophysiology data acquisition & analysis software.  If you would like more information regarding this equipment, please contact Dr. David Estrada, Assistant Professor of Materials Science & Engineering at daveestrada@boisestate.edu or (208) 426-5693.

Histology Equipment

Leica TP1020 150pxThe Leica TP1020 Benchtop autoembedder is a benchtop tissue processor designed to provide gentle dehydration, clearing and infiltration of various tissues with paraffin wax.  The TP1020 is completely programmable, allowing tissue-specific programs to be developed.  A programmable, optional vacuum setting is available to improve infiltration.


Leica EG1150 150pxThe Leica EG1150H is a heated paraffin dispensing module with a large heated work surface and storage areas for both cassettes and molds.  The embedding module aids in obtaining proper orientation of tissues and final embedding in blocks of paraffin for sectioning.


Leica RM2235 150px

The Leica RM2235 Rotary Microtome is designed for precision manual sectioning of paraffin embedded tissues.  The BRC has a knife holder for low profile blades, and a separate knife holder for high profile blades, used for sectioning harder materials.  This microtome has a sectioning range of 1-60 mm.


Leica CM1950 150px x 150px

The Leica CM1950 cryostat is available for safe, accurate and fast high quality sectioning.  The cryostat can section frozen tissues up to 100 microns in thickness.


Leica VT1000 150pxThe Leica Vibratome VT1000 is allows sectioning of fresh or fixed tissue with or without embedding in support media such as agarose.  Speed, frequency, amplitude, and clearance angle are all easily adjustable, and sections can be cut up to 999 microns.


Leica autostainer XL 150pxThe Leica Autostainer XL provides a gentle method of staining multiple slides.  This system is currently set up to quickly and easily stain tissues with hematoxylin and eosin and/or to simply dewax slides cut from paraffin blocks. The processor has the capability to automate multiple staining protocols, with up to 15 programs of 25 steps each.


Leica DM1000 150px x 150pxThe Leica DM 1000 LED transmitted light microscope features long-life LED illumination that provides near daylight, bright illumination with constant color temperature.  This is an extremely versatile microscope system, and can be optionally equipped for dark field, phase contrast, polarization contrast, and fluorescence.  The microscope is fitted with a third generation SPOT TM RT3 Slider camera, which allows the user to capture both true color imaging and high sensitivity monochrome images.


Pathscan enabler IV 150pxThe PathScan Enabler IV Slide Scanner is designed to allow the scanning of an entire whole-mounted histology sample attached to a standard 1 x 3 inch cover-slipped glass slide.  This allows for the production of low power, large field of view, high resolution imaging.  Pathscan produces image files that can be saved in a variety of formats including TIFF and JPEG.


Please contact Dr. Keller-Peck at 208-426-2254 or by email at ckpeck@boisestate.edu  for more information.

Imaging

Zeiss LSM Meta 510The Biomolecular Research Center’s Zeiss Confocal Microscope, LSM 510 Meta coupled with an inverted Axiovert Observer Z1 complete with high NA objective lens from 5x to 100x that include both water and oil immersion, is configured with a 405 nm diode and six laser lines (405, 458, 488, 514, 543 and 633nm), two standard and a spectral detector.  Zeiss LSM 510 system accessories include a heated stage and objective warmer.


SkyScan 1172The BRC’s SkyScan 1172 High resolution X-ray Micro-CT Scanner  utilized for scanning high-density tissues such as bones to low-density softer tissue such as lung. The Skyscan 1172 with dynamically variable acquisition geometry provides the shortest scan times possible at any magnification and cross-section images are generated in a wide range of formats up to 8000 x 8000 pixels. 2D/3D reconstruction can be performed using a single PC or using the 4-PC cluster in the BRC facility to enhance post-scan reconstructions times


Zeiss StemiSV1 M2BIOThe Zeiss StemiSV11M2 Bio Quad Fluorescent Microscope is designed in collaboration with Kramer Scientific, M2 Bio Quad uniquely combines stereo macro and compound micro imaging capabilities in one microscope. This ability to combine the ergonomics of stereo observation and high-resolution microscopic observation in a single microscope offers enormous improvements in time management in the exploding field of studying fluorescent proteins of GFP, YFP, BFP and its new counterparts. Now you can sort larger populations in stereoscopic mode and simply pivot the objective system into position for high-magnification detailed observation.


Olympus America's BX53The Olympus America’s BX53 is a compound microscope with an automated mechanical stage, camera, computer interface, digital display, fine focus, and image analysis software and oil immersion lens. Samples can be viewed by brightfield, phase contrast and under polarizing light. Objective magnification from 1.25x to 40X.


AMG EVOSAMG EVOS Fluorescence microscope is an easy to use fully integrated inverted multichannel LED fluorescent microscope capable of screening slides stained with DAPI, green and red fluorescence along with phase contrast imaging. Software includes tools for overlaying images and counting. The EVOS microscope has a manual stage with many adapters allowing slides, multi-well wells, 100mm and 35tmm dishes and flasks to be easily observed and imaged.


Biorad Pro FX ImagerThe BioRad Pro Fluorescent Phospho-Imager is optimally suited for the field of proteomics, this fluorescence scanner has 50-μm resolution and a 5 order of magnitude linear dynamic range is capable of single or multicolor fluorescent scanning, can accommodate large gels and images are integrated with PDQuest 2-D Analysis Software. Internal and external lasers allow this unit to perform digital autoradiography by scanning phosphor plates or performing corresponding tissue fluorescence imaging by directly scanning thin tissue sections.


Kodak Imger 4000RThe Kodak Imager 4000R imaging system has cooled CCD imaging technology, 4-million pixel resolution, true 16-bit imaging producing 65,000 levels of grayscale resolution for accurate intensity measurement. Detection capabilities include absorbance, chemiluminescence and UV fluorescence imaging.


Luminex 100The Luminex 100 is a compact lab analysis system based on the principles of flow cytometry which enables the simultaneous assay of up to 100 analytes in a single well of a microtiter plate with very small volumes. This system delivers fast and cost-effective bioassay results on many assay formats including nucleic-acid assays, receptor-ligand assays, immunoassays and enzymatic assays.


SynergyMx plater readerThe SynergyMx is a flexible and easy to use monochromator-based multi-mode microplate reader with fluorescence, absorbance and luminescence detections modes. The monochromatic optics can select any UV-Visible wavelength in 1nm increments.


Zeiss Axiovert CFLThe Zeiss Axiovert 40CFL for transmitted light, Phase contrast, DIC and fluorescence, fixed camera port connected to Zeiss ERc5S Zeiss digital camera, equipped with 5X, 10X, 20X and 40X objectives and filters for common blue and green fluorescence.


PupilCamThe Pupil Cam is an easy-to-use digital microscope camera with a built-in 10x eyepiece that gets placed directly on the ocular tube of the most standard microscopes. High resolution images can capture full screen microscopic images. These images can then be manipulated within the software. Pre-attached USB or Video cables make for easy attachment to your computer or monitor.


AxioCam ERc5sThe AxioCam ERc 5s microscope camera can be operated via AxioVision in the conventional way, but it also works as a stand-alone imaging station. Neither a PC nor any software are required in order to acquire images of your samples. This ZEISS camera acquires color images with 5 megapixels and stores them on memory cards in the economical and widely used SD format. The AxioCam ERc 5s is operated simply via two easily accessible buttons: one triggers the acquisition of the image, while the other performs the automatic white balance. If you subsequently wish to transfer your images to a PC, all you need to do is insert the SD card into the memory card slot on your PC.


Pentax Optio W80The Pentax Optio W80 is an ultra durable shock resistant waterproof camera up to depths of 16 feet in water where it can take underwater video. It is also resistant to temperatures of -10ºC so is well suited for taking images  in the field. While only an inch thin, it has a 12.1 megapixel sensor with a 5X optical, Super Macro mode which brings details in from even the smallest and closest subjects (as close as 1cm).


Gel Logic 200

The Gel Logic 200 system features great imaging capacity.  A UV transilluminator, and protective UV shield, makes visualization and excision of bands from ethidium bromide and other fluorescent dyes, simple and safe.  White light illumination capability with a UV conversion screen and while light epi-illumination for visualization of samples labeled with chromogenic labels. This unit is equipped with real time viewing for sample positioning and adjustments and a mega-pixel scientific grade CCD sensor that captures crisp detailed images increasing the quantitative accuracy of analyses.


FluorChem E ProteinScienceThe FluorChem E Digital Darkroom is designed for high-performance western blot and gel imaging able to detect chemiluminescent, colorimetric and UV fluorescent gels and blots. It includes a state-of-the-art CCD optical technology with simplified touch screen allowing users to select settings, capture images, save and print images all from one screen.


PathScan Enabler IVThe PathScan Enabler IV created the market for personal digital slide scanning. With an innovative and intuitive design, the PathScan Enabler IV allows scanning of whole mounted histological sample attached to standard 1 X 3 inch cover slipper glass slides. The PathScan Enabler IV solves low power, large field of view, high resolution imaging problems. The scan area is 1.423 X 0.85 inches, resulting in stunning, incredible images up to 10,248 x 6120 pixels! The PathScan Enabler IV holds two (2) 1 X 3 inch glass slides and includes simple use one touch QuickScan software, as well as SilverFast Ai Software for advanced users. Image files are saved in TIFF or JPEG formats. JPEG, GIF, etc.


 Countess® II FL Automated Cell CounterThe new Countess® II FL Automated Cell Counter is a benchtop cell assay platform equipped with state-of-the-art optics and image analysis software for rapid assessment of cells in suspension. Now with fluorescence capabilities—bright-field and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, and measure cell viability.


FluorChem RThe FluorChem R is a simple to use and multifaceted Imaging Station designed with an integrated computer, intuitive touch-screen and outfitted with state-of-the-art CCD optical technology. This system has the capability to image data from multiplex, chemiluminescent, and colorimetric western blots as well as from UV fluorescent DNA and protein gels. Post-capture image optimization and analysis can be performed via a web browser accessible from image station, PC, MAC, tablet or smartphone.


TS100 inverted microscope & SPOT RT3 CameraThe TS100 inverted microscope & SPOT RT3 Camera. The microscope utilizes a high-intensity LED eco-illumination system reducing eyestrain for users and eliminates color temperature changes that occur with halogen light sources. The SPOT RT3 microscope camera is easy to use, able to capture both color or monochrome images, and excellent for both brightfield and fluorescence imaging.


Olympus BX53-P Polarizing Microscope The Olympus BX53-P Polarizing Microscope performs superbly in polarized light. It’s a breakthrough combination of UIS2 infinity-corrected optics and Olympus original optical-design. Olympus has also devised a new, extended line of compensators to make the BX53-P versatile enough to handle observation and measuring applications in virtually any field. Thanks to Olympus’ sophisticated design and manufacturing technology, the ACHN-P and UPLFLN-P objectives reduce internal strain. BX3 polarizing microscope features high EFvalue filters in polarizer and analyzer resulting in unmatched image contrast. To meet a diversity of research and application requirements, the universal UPLFLN-P series objectives have been designed to accommodate a wide range of observation methods, including Nomarski DIC and fluorescence microscopy, in addition to polarized light observation.


ivisspectrumAn IVIS® Spectrum is available for optical imaging technology of mice to facilitate non-invasive longitudinal monitoring of disease progression. A Skyscan 1172 High Resolution X-ray Micro-CT Scanner is also available for bone analysis.


StatSpin CytoFuge The StatSpin CytoFuge provides a simple solution to preparing thin-layer cell preparations. Suspended cell suspensions are processed quickly onto a microscope slide with filter concentrators. Results are consistent, easy to scan monolayer cell presentations with morphologic detail. The Cytospin has six separate time cycles and speed settings from 600-4400 rpm.

 

 

Mass Spectrometry

Velos LIT_2Themo Scientific Velos Pro LIT Mass Spectrometer is an ion trap mass spectrometer and it is located in the BRC, room 215 in the Mathematics Building at Boise State University . It offers Trap-HCD (Higher-Energy Collisional Dissociation) combined with CID (Collision-Induced Dissociation), and PQD (Pulsed-Q Dissociation) to enhance coverage and sensitivity of proteomic analysis. An Easy nL nano liquid chromatographic system is coupled to the mass spectrometer through the nanoelectrospray source for protein characterization. In combination with the Thermo Proteome Discover 1.3 and Sequest and Mascot database search engine, this LC-MS system is our work horse for routing proteomic analysis.


Bruker QTOFBruker Q-TOF Mass Spectrometer is located in the mass spectrometer is located in the BRC, room 215 in the Mathematics Building on the campus of Boise State University.  This system is a hybrid tandem mass spectrometer with outstanding performance including fast acquisition rate (up to 30 Hz for small molecules, up to 5Hz dynamic for peptides), high resolution (50,000 Full Sensitivity and Resolution), high resolution EIC (0.5 – 1mDa on typical LC peaks), and  excellent sensitivity (1 pg Reserpine >100:1 S/N RMS). This mass spectrometer is coupled with a Dionex Ultimate 3000 HPLC system and an innovative Captive electrospray source. In combination with software tools, including Bruker Compass Data Analysis, Smartformula, ProteinScape, Mascot protein search engine, and Profile Analysis, we use this LC-MS system in small molecule identification, metabolomics analysis and protein characterization.


Bruker HCTBruker HCT Ion Trap Mass Spectrometer from Bruker Daltonics is located in the Analytical Lab, room 363 in the Science building on the Boise State University campus. The HCT is a robust and easy to use mass spectrometer. Coupled with an electrospray sources, it provides good MS and MS/MS spectra quality – in terms of mass resolution, sensitivity and accuracy throughout a wide mass range for applications like: Protein identification, metabolite characterization, and general chemistry LC/MSn. Equipped with an Electron-transfer dissociation (ETD) and CID, HCT provides unique capability to identify post translational modification of protein.


Trace GC/MSn UltraThe Thermo Polaris Q GC/MSn Benchtop Ion Trap Mass Spectrometer complete with Thermo Trace Gas Chromatograph, external vacuum pump, and XCalibur PC workstation. This combination of power of the MS ion trap technology target specific compounds in complex matrices. The Polaris Q offers unparalleled sensitivity in full scan, plus the power of MS/MS, the PolarisQ provides dependable performance for routine application plus the added horsepower to go further enabling lower detection in complex matrices.


Genius 1050- Nitrogen Generator Utilizing Carbon Molecular Sieve and Pressure Swing Adsorption, the Genius 1050 provides on-site nitrogen for your LC-MS instrument. The Genius 1050 is well suited for a range of particularly demanding applications where specific performance specifications are required.

 


TM -Sprayer HTX Imaging’s patented TM-Sprayer™ Tissue MALDI Sample Preparation System is designed to provide the highest quality matrix deposition on the two-dimensional biological samples, and can be integrated with any MALDI Mass Spectrometer platform. Because the principle of MALDI MS is based on the introduction of a UV-activated matrix molecule in the immediate proximity of the analyte, it is imperative to control the process by which matrix is introduced in the sample. Unique in design, the TM-Sprayer provides heat to the matrix solution to accelerate adsorption into the tissue, as well as a controlled flow of dry air or nitrogen to focus the spray and control drying time.
Using the TM-Sprayer™ the matrix solution is both heated and pressurized, which gives the matrix spray several beneficial properties:

• The heated matrix is able to better penetrate the tissue surface and reach analytes.
• The faster rate of evaporation avoids the creation of large matrix crystals (often known to create hot spots).
• The pressurized spray is very directional, ensuring a precise location of the matrix deposition.
• The combined effect of the nozzle velocity and sheath gas flow (dry air or nitrogen can be used) allows for controlled drying speed.

Molecular Biology

Thermo Hybridization Oven
The Thermoscientific Hybridization Oven has a 10-bottle capacity rotisserie with an accurate and uniform temperature control and uniformity. Temperature Range from 8 to 85 °C, temperature uniformity within bottle is ±0.25 °C and rotisserie speed from 5 to 15 rpm.


ThermoMix500The ThermoMix 500 is a digitally controlled Peltier heater and cooler (6C – 105 C) orbital mixer (300-1500 rpm) for cell killing assays, RNA transcription, immunoprecipitation, enzyme kinetics, ligations, and more. Five interchangeable sample blocks are available for plates and different size tubes.


Nanodrop ND-1000The Nanodrop ND-1000 is sample retention system designed to measure small volume samples of nucleic acids and proteins without the use of cuvettes or capillaries. Absorbance is captured and measured in a sample column made between two optical pedestals, displayed on an easy to read software interface, collected in an exportable spreadsheet and stored within the computer’s hard-drive. Measures DNA, RNA (A260) and Protein (A280) concentrations and sample purity (260/280 ratio); Large concentration range (2 ng/µL – 15,000 ng/µL dsDNA) without dilutions. Nanodrop software is pre-configured methods for common applications such as Nucleic Acid, Protein A280, Microarray, Proteins & Labels, Bradford, BCA, Lowry and Pierce 660nm.


ABI7300The ABI 7300 Real Time PCR System combines thermal cycling, fluorescence detection and application specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube reaction. The ABI utilizes a 96 well format supporting a volume range of 20-100 µl. Four-color detection provides the flexibility to perform a variety of applications which include gene expression analysis, pathogen quantification and SNP genotyping. Software includes plate setup wizard, advanced data viewing, sorting and graphing capabilities with quantitative results that are available immediately upon completion of PCR.


SmartCycler Real Time PCrThe Smart Cycler Real Time PCR machine from Cepheid utilizes the I-CORE module of microfluidic and microelectronic design. Each Smart Cycler processing block contains 16 independently programmable I-CORE modules, each of which performs four-color, real-time fluorometric detection. A variety of simplex or multiplex fluorescent tags can ne used in conjunction with this system including FAM, TET< ALEXA 532m ALEXA 748, TAM, ROX, SYBE Green, Cy3, Texas Red and others.


Veriti Thermal CyclerThe Veriti Thermal Cycler from Applied Biosystems has six independent temperature blocks which adds increased flexibility and precision ideal for PCR optimization and the comprehensive gene amplification. The Veriti has an easy touch screen user interface. The GeneAmp 2720Thermal Cycler, also from Applied Biosystems, is designed for a full range of PCR and cycle sequencing applications. Both the Veriti and the GeneAmp 2720 thermal cycler units are equipped with a 96-well format compatible with standard 0.2ml PCR tubes and 96 well reaction plates.


FLUXION BIOSCIENCES BIOFLUX CONTROLLER The BioFlux System gives you the ability to introduce flow to your research and drug discovery experiments effectively emulating in vivo conditions and getting closer to understanding what’s happening under more physiological conditions. Offering the physiological relevance of laminar flow chambers with the throughput and convenience of standard well plates, BioFlux systems have many features and benefits including:
• High Speed: With automated experimental control, multiplexing up to 96 simultaneous experiments, and sophisticated data analysis software simplifying and accelerating complex functional assays, large projects can be effectively reduced from months to days.
• High Quality: Glass bottom plates with superior image quality from microscopes and high content imagers provide unparalleled image quality.
• Simple to Use: Well plate microfluidic design improves reliability and reproducibility while enabling precise control of critical parameters.
• Modular Design: A BioFlux system can be added to any existing inverted microscope setup instantly expanding its capabilities.


2100 Electrophoresis Bioanalyzer The Agilent 2100 Bioanalyzer system provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform, providing high quality digital data.

• Two assay principles – electrophoresis and flow cytometry
• Ready-to-use assays and pre-packaged reagent kits
• Minimal sample consumption (1-4 µL)
• Easy to use – load the chip, press “start“, the instrument does the rest!
• Digital data for convenient analysis, archiving and storage
• Contamination-free switch of methods

Protein Interaction and Determination

VarianCary50The Varian Cary 50 UV/VIS Spectrophotometer has a data collection rate of 80 points per second that can be optimally utilized to acquire information for kinetics assays. This unit features a maximum scan rate of 24, 000 nm per minute and a scan range for 190-1100 that can be completed in less than 3 seconds. The use of a Xenon lamp which flashes only when acquiring a data point minimizes the degradation of photosensitive samples, and as the Xenon lamp is intense, a beam splitter can be used with the loss in energy and allows for simultaneous beam correction.


Reichart SR7000 SPRReichert SR7000 Surface Plasmon Resonance spectrometer is designed for label-free, real-time detection of molecular interactions with nano to micro to macro applications. This instrument monitors surface plasmon resonance (SPR) and offers critical angle capability, and monitors response versus reflectivity data. This Reichart SPR has a high thermal conductivity, works in a temperature range of 10 °C to 90 °C, is easily programmable and can has kinetic capability over a broad temperature range. This system has been automated with the addition of a Themofinnagen Surveyor Auto Sampler and Pump.


Varian Cary 100 UV-Vis SpectrophotometerThe Varian Cary UV-Vis Spectrophotometer is an instrument that utilizes the visible and UV portion (190 nm to ~900nm) of the electromagnetic spectrum to monitor the electronic transitions of molecules as the monitoring of absorption peaks can be correlated with the types of bonds and valuable in determining the functional groups within a molecule. This spectrophotometer is equipped with a Petlier-thermostatted mulitcell sample transport, a temperature controller, an extended sample compartment, Cary UVWin Bio software, and includes a variety of modules: Simple and Advanced Reads module, Concentration and Kinetics modules, Enzyme and Scanning modules, and RNA/DNA and renaturation modules, a  self-contained unit which drives Peltier heating in the thermostable accessory which is attached and regulated by a built-in radiator with circulating water.


Cary Eclipse FluorimeterThe Cary Eclipse Fluorescence Spectrophotometer uses a Xenon flash lamp for superior sensitivity, high signal-to-noise, and fast kinetics. It measures the emission of light from samples in four modes. Using Xenon lamp technology, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts. The Cary Eclipse is the only spectrophotometer with room light immunity. With an optional microplate reader, it offers full wavelength scanning of 384 samples.


SynergyMx plater readerThe Biotek SynergyMx is a flexible and easy to use monochromator-based multi-mode microplate reader with fluorescence, absorbance and luminescence detections modes. The monochromatic optics can select any UV-Visible wavelength in 1nm increments.


Biotek Synergy HTThe BioTek  Synergy™ HT Multi-Mode Microplate reader designed  with a dual optics design for fluorescence, absorbance and luminescence measurements.  With a tungsten halogen lamp, this unit provides constant intensity, increased sensitivity and repeatability. This system has the ability to use filters with different bandpasses which allows the investigator to tailor their fluorescence measurements to their experiment more effectively.


Jasco J810The Jasco J-810 spectropolarimeter is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer. It is designed to allow secondary structure determination by circular dichroism; specifically measuring the left-and right-handed circularly polarized light of optically active molecules. It is equipped with a thermoelectric type temperature control system and flo-through HPLC cell. The instrument is controlled by Jasco’s Spectra Manager software.


XL-I analytical UltracentrifugeThe ProteomeLab/XL-I analytical ultracentrifuge measures the relative change in the distribution of molecular weights, providing an efficient way to measure heterogeneity, stoichiometry, and self-associating systems.  It is designed for the in-solution characterization of proteins, oligomers, aggregates, particles, colloids, and small structures. The wide range of rotational velocities also allows investigators to probe protein size, dimerization, and binding constants. Sedimentation velocity experiments are utilized to determine protein molecular weight and identify degree of dimerization and sedimentation equilibrium experiments are utilized to determine binding constants.

Real-Time and Quantitative PCR

ABI7300The ABI 7300 Real Time PCR System combines thermal cycling, fluorescence detection and application specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube reaction. The ABI utilizes a 96 well format supporting a volume range of 20-100 µl. Four-color detection provides the flexibility to perform a variety of applications which include gene expression analysis, pathogen quantification and SNP genotyping. Software includes plate setup wizard, advanced data viewing, sorting and graphing capabilities with quantitative results that are available immediately upon completion of PCR.


SmartCycler Real Time PCrThe Smart Cycler Real Time PCR machine from Cepheid utilizes the I-CORE module of microfluidic and microelectronic design. Each Smart Cycler processing block contains 16 independently programmable I-CORE modules, each of which performs four-color, real-time fluorometric detection. A variety of simplex or multiplex fluorescent tags can ne used in conjunction with this system including FAM, TET< ALEXA 532m ALEXA 748, TAM, ROX, SYBE Green, Cy3, Texas Red and others.


Veriti Thermal CyclerThe Veriti Thermal Cycler from Applied Biosystems has six independent temperature blocks which adds increased flexibility and precision ideal for PCR optimization and the comprehensive gene amplification. The Veriti has an easy touch screen user interface. The Veriti is a thermal cycler is equipped with a 96-well format compatible with standard 0.2ml PCR tubes and 96 well reaction plates.


GeneAmp 2720The GeneAmp 2720Thermal Cycler, also from Applied Biosystems, is designed for a full range of PCR and cycle sequencing applications. The GeneAmp 2720 is a thermal cycler is equipped with a 96-well format compatible with standard 0.2ml PCR tubes and 96 well reaction plates.


Mastercycler ep realplexThe Mastercycler ep realplex is designed to meet all the requirements of quantitative PCR applications which includes extremely fast temperature ramping, short detection times and intuitive assay programming translate to huge time-savings so that more experiments can completed per day-always with accurate and reliable results. It′s flexibility is beyond compare-this completely open system allows you to use tubes, plates and reagents of your choice. No other real-time cycler on the market today offers such freedom.


Mastercycler ep realplexThe Roche’s Light Cycler 96 utilizes leading-edge fixed fiber optics to provide simultaneous and therefore unbiased real-time data capture from one, and up to four, channels. This light cycler was designed with both four excitation and four emission filters, with a 10-50μl reaction volume, to accommodate a sample format of an 8-tube strip or 96 well plates and with detection formats that include intercalating dyes and hydrolysis probes.

Tissue and Cell Culture

Nikon-TC-scope-pic The TS100 inverted microscope & SPOT RT3 Camera. The microscope utilizes a high-intensity LED eco-illumination system reducing eyestrain for users and eliminates color temperature changes that occur with halogen light sources. The SPOT RT3 microscope camera is easy to use, able to capture both color or monochrome images, and excellent for both brightfield and fluorescence imaging.


Countess® II FL Automated Cell CounterThe new Countess® II FL Automated Cell Counter is a benchtop cell assay platform equipped with state-of-the-art optics and image analysis software for rapid assessment of cells in suspension. Now with fluorescence capabilities—bright-field and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, and measure cell viability.


PathScan Enabler IVThe PathScan Enabler IV created the market for personal digital slide scanning. With an innovative and intuitive design, the PathScan Enabler IV   allows scanning of whole mounted histological samples attached to standard 1 X 3 inch cover slipper glass slides. The PathScan Enabler IV solves low power, large field of view, high resolution imaging problems. Scan area is 1.423 X 0.85 inches, resulting in stunning, incredible images up to 10,248 x 6120 pixels! The PathScan Enabler IV holds two (2) 1 X 3 inch glass slides and includes simple use one touch QuickScan software, as well as SilverFast Ai Software for advanced users. Image files are saved in TIFF or JPEG formats. JPEG, GIF, etc.

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