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Mass Spectrometry

Velos LIT_2Themo Scientific Velos Pro LIT Mass Spectrometer is an ion trap mass spectrometer and it is located in the BRC, room 215 in the Mathematics Building at Boise State University . It offers Trap-HCD (Higher-Energy Collisional Dissociation) combined with CID (Collision-Induced Dissociation), and PQD (Pulsed-Q Dissociation) to enhance coverage and sensitivity of proteomic analysis. An Easy nL nano liquid chromatographic system is coupled to the mass spectrometer through the nanoelectrospray source for protein characterization. In combination with the Thermo Proteome Discover 1.3 and Sequest and Mascot database search engine, this LC-MS system is our work horse for routing proteomic analysis.


Bruker QTOFBruker Q-TOF Mass Spectrometer is located in the mass spectrometer is located in the BRC, room 215 in the Mathematics Building on the campus of Boise State University.  This system is a hybrid tandem mass spectrometer with outstanding performance including fast acquisition rate (up to 30 Hz for small molecules, up to 5Hz dynamic for peptides), high resolution (50,000 Full Sensitivity and Resolution), high resolution EIC (0.5 – 1mDa on typical LC peaks), and  excellent sensitivity (1 pg Reserpine >100:1 S/N RMS). This mass spectrometer is coupled with a Dionex Ultimate 3000 HPLC system and an innovative Captive electrospray source. In combination with software tools, including Bruker Compass Data Analysis, Smartformula, ProteinScape, Mascot protein search engine, and Profile Analysis, we use this LC-MS system in small molecule identification, metabolomics analysis and protein characterization.


Bruker HCTBruker HCT Ion Trap Mass Spectrometer from Bruker Daltonics is located in the Analytical Lab, room 363 in the Science building on the Boise State University campus. The HCT is a robust and easy to use mass spectrometer. Coupled with an electrospray sources, it provides good MS and MS/MS spectra quality – in terms of mass resolution, sensitivity and accuracy throughout a wide mass range for applications like: Protein identification, metabolite characterization, and general chemistry LC/MSn. Equipped with an Electron-transfer dissociation (ETD) and CID, HCT provides unique capability to identify post translational modification of protein.


Trace GC/MSn UltraThe Thermo Polaris Q GC/MSn Benchtop Ion Trap Mass Spectrometer complete with Thermo Trace Gas Chromatograph, external vacuum pump, and XCalibur PC workstation. This combination of power of the MS ion trap technology target specific compounds in complex matrices. The Polaris Q offers unparalleled sensitivity in full scan, plus the power of MS/MS, the PolarisQ provides dependable performance for routine application plus the added horsepower to go further enabling lower detection in complex matrices.


Genius 1050- Nitrogen Generator Utilizing Carbon Molecular Sieve and Pressure Swing Adsorption, the Genius 1050 provides on-site nitrogen for your LC-MS instrument. The Genius 1050 is well suited for a range of particularly demanding applications where specific performance specifications are required.

 


TM -Sprayer HTX Imaging’s patented TM-Sprayer™ Tissue MALDI Sample Preparation System is designed to provide the highest quality matrix deposition on the two-dimensional biological samples, and can be integrated with any MALDI Mass Spectrometer platform. Because the principle of MALDI MS is based on the introduction of a UV-activated matrix molecule in the immediate proximity of the analyte, it is imperative to control the process by which matrix is introduced in the sample. Unique in design, the TM-Sprayer provides heat to the matrix solution to accelerate adsorption into the tissue, as well as a controlled flow of dry air or nitrogen to focus the spray and control drying time.
Using the TM-Sprayer™ the matrix solution is both heated and pressurized, which gives the matrix spray several beneficial properties:

• The heated matrix is able to better penetrate the tissue surface and reach analytes.
• The faster rate of evaporation avoids the creation of large matrix crystals (often known to create hot spots).
• The pressurized spray is very directional, ensuring a precise location of the matrix deposition.
• The combined effect of the nozzle velocity and sheath gas flow (dry air or nitrogen can be used) allows for controlled drying speed.